Formulation of Insect Chitosan Stabilized Silver Nanoparticles with Propolis Extract as Potent Antimicrobial and Wound Healing Composites

Skin wounds are frequently influenced with microbial infections and inflammation, which need innovative agents for disputing them. Chitosan (Csn) was extracted from larvae of BSF “black soldier fly, Hermetia illucens” ethanolic propolis extract (Pro) employed synthesizing silver nanoparticles (Ag-NPs), using facile biogenic protocol. The BSF-Csn acquired a yield 1.56%, 91.3% deacetylation degree, 88.600 Dalton molecular weight. Ag-NPs were effectually biosynthesized Pro, mean diameter 8.73 nm zeta potential -21.34 mV. antimicrobial activities assessment insect Csn, synthesized their composite against skin pathogens (Staphylococcus aureus Candida albicans) revealed the elevated efficiency individual superior action (Csn/Pro/Ag-NPs), 26.3 23.4 mm inhibition zones inhibitory concentrations 35.0 45.0 μg/mL toward S. C. albicans, respectively, exceeded actions commercial antibiotics. treatment rat’s this promisingly led to faster healing absence inflammation infection signs. powerful Csn/Pro/Ag-NPs as wound strongly advocate applications protection, disinfection, regeneration.